A Soluble C1b Protein and Its Regulation of Soluble Type 7 Adenylyl Cyclase†

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A soluble C1b protein and its regulation of soluble type 7 adenylyl cyclase.

Adenylyl cyclase (AC) is a prototypical cell-signaling molecule expressed in virtually all organisms from bacteria to man. While C1b, a poorly conserved region within mammalian AC, has been implicated in numerous isoform-specific regulatory properties, no one has purified the C1b region as a functional protein to homogeneity in order to study its role in enzyme function. We hypothesize that C1b...

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Mammalian membrane-bound adenylyl cyclase consists of two highly conserved cytoplasmic domains (C1a and C2a) separated by a less conserved connecting region, C1b, and one of two transmembrane domains, M2. The C1a and C2a domains form a catalytic core that can be stimulated by forskolin and the stimulatory G protein subunit alpha (Galpha(s)). In this study, we analyzed the regulation of type 7 a...

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ژورنال

عنوان ژورنال: Biochemistry

سال: 2004

ISSN: 0006-2960,1520-4995

DOI: 10.1021/bi049088+